Analytical performance of species-targeted quantitative PCR for intra-abdominal candidiasis in critically ill patients: a proof-of-concept post hoc analysis from the pBDG2 multicenter study
Authors' conclusion
OLM CandID Real-Time PCR applied directly to peritoneal fluid demonstrated strong analytical performance for the detection of Candida in critically ill patients with suspected IAC.
Study context
- Population
- Critically ill patients with suspected or confirmed intra-abdominal candidiasis, mainly postoperative peritonitis (pBDG2 multicentre cohort)
- Participants
- 57 patients
- Comparison
- OLM CandID Real-Time PCR on peritoneal fluid vs fungal culture
- Outcome
- Diagnostic performance of binary PCR results vs fungal culture
Methodological detailsproof-of-concept, post hoc analysis, multicentre
- Proof-of-concept
- Post hoc analysis
- Multicentre
Performance
As reported in the abstract
Diagnostic accuracy of binary PCR vs fungal culture (reference standard)
Sensitivity
100%
95% CI 100–100
Specificity
60%
95% CI 38–81
PPV
82%
95% CI 71–93
NPV
100%
95% CI 100–100
Further findings
Cycle threshold (Ct)-based ROC analysis, C. albicans (subgroup)
- ROC AUC
- 0.92 (95% CI 0.75-1)
- Optimal Ct cutoff
- 30.75
- Specificity at optimal Ct cutoff
- 100%
- PPV at optimal Ct cutoff
- 100%
- n = 35
- Secondary analysis
Ct-based ROC analysis, C. tropicalis (subgroup)
- ROC AUC
- 0.90 (95% CI 0.66-1)
- Optimal Ct cutoff
- 34.63
- Specificity at optimal Ct cutoff
- 100%
- PPV at optimal Ct cutoff
- 100%
- n = 12
- Secondary analysis
Original publication
Analytical performance of species-targeted quantitative PCR for intra-abdominal candidiasis in critically ill patients: a proof-of-concept post hoc analysis from the pBDG2 multicenter study. Crit Care. Published 10 February 2026. doi:10.
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