Consistency between metagenomic next-generation sequencing versus traditional microbiological tests for infective disease: systemic review and meta-analysis
Authors' conclusion
This review demonstrates a moderate relationship between mNGS and traditional pathogen tests, indicating a complex relationship between these two methods. Sterile samples show higher consistency than non-sterile samples. Immune function deficiency may reduce the consistency between mNGS and traditional tests.
Study context
- Population
- Patients with infective disease (sepsis and septic shock context)
- Participants
- 4,112 patients · 27 studies
- Comparison
- Metagenomic next-generation sequencing (mNGS) vs traditional microbiological tests
- Outcome
- Kappa consistency between mNGS and traditional pathogen tests
Methodological detailsQUADAS-2 quality assessment
- QUADAS-2 quality assessment
Pooled result
As reported in the abstract
Pooled kappa consistency
Pooled consistency, moderate relationship (p < 0.001)
0.319 ± 0.013
Further pooled results
Kappa consistency, cerebrospinal fluid samples (subgroup)
- Highest pooled kappa consistency among sample types (p < 0.001)
- 0.500 ± 0.029
Kappa consistency by immune status (subgroup)
- Immunocompromised patients (p < 0.001)
- 0.294 ± 0.014
- Immunocompetent patients (p < 0.001)
- 0.321 ± 0.028
Percent agreement of mNGS vs traditional tests
- Positive percent agreement
- 83.63%
- Negative percent agreement
- 54.59%
Original publication
Consistency between metagenomic next-generation sequencing versus traditional microbiological tests for infective disease: systemic review and meta-analysis. Crit Care. Published 3 February 2025. doi:10.
Composed by AI from the published abstract and checked against it. How Briefs are made →