Microbiome Profiling Demonstrates Concordance of Endotracheal Tube Aspirates With Direct Lower Airway Sampling in Intubated Patients
Authors' conclusion
Unbiased molecular profiling shows that standard clinical ETA sampling has good concordance with the authentic lower airway microbiome in intubated patients.
Study context
- Population
- Intubated patients undergoing percutaneous tracheostomy following prolonged (median, 15 days) intubation
- Participants
- 13 patients
- Comparison
- ETA vs Tracheostomy
- Outcome
- Concordance of bacterial composition, diversity and relative quantification between ETA and lower airway microbiome
Methodological details16S rRNA V1-V2 gene sequencing, contemporaneous gold standard sampling
- 16S rRNA V1-V2 gene sequencing
- Contemporaneous gold standard sampling
Performance
As reported in the abstract
Bacterial composition, diversity and quantification
- Pre-tracheostomy endotracheal aspirates (ETAs) closely resembled gold standard post-tracheostomy airway microbiomes in composition, diversity and quantification
Further findings
Endotracheal tube and suction catheter biofilms
- Biofilms resembled cognate ETA and fresh tracheostomy communities
Original publication
Microbiome Profiling Demonstrates Concordance of Endotracheal Tube Aspirates With Direct Lower Airway Sampling in Intubated Patients. Chest. Published 9 January 2024. doi:10.
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