Whole blood transcriptomics identifies subclasses of pediatric septic shock
Authors' conclusion
Two subclasses of pediatric septic shock patients were discovered through genome-wide expression profiling based on whole blood RNA sequencing with major biological and clinical differences.
Study context
- Population
- Critically ill children with cardiopulmonary failure in a randomised insulin titration trial to treat hyperglycemia; children with septic shock46 septic shock, 52 controls
- Comparison
- Subclass 1 vs Subclass 2 of pediatric septic shock
- Controlsn = 52
- Primary endpoint
- Identification of pediatric septic shock subclasses based on whole blood RNA expression profiles
Methodological detailsobservational, genome-wide expression profiling (RNA sequencing), hierarchical clustering
- Observational
- Genome-wide expression profiling (RNA sequencing)
- Hierarchical clustering
Primary result
As reported in the abstract
Septic shock subclasses
- Two subtypes identified among patients with septic shock based on gene expression patterns
- Subclass 1 vs Subclass 2: upregulation of innate and downregulation of adaptive immunity pathways
- Subclass 1 had significantly worse clinical outcomes despite similar illness severity on initial presentation
- Subclass 1: elevated plasma inflammatory cytokines and endothelial injury biomarkers, decreased % CD4 T cells and B cells, less diverse T cell receptor repertoires
Further findings
Septic shock vs controls
- Alterations in innate and adaptive immune pathways in septic shock
Original publication
Whole blood transcriptomics identifies subclasses of pediatric septic shock. Crit Care. Published 8 December 2023. doi:10.
Composed by AI from the published abstract and checked against it. How Briefs are made →