Endothelium-Derived Extracellular Vesicles Expressing Intercellular Adhesion Molecules Reflect Endothelial Permeability and Sepsis Severity
Authors' conclusion
EDEVs expressing intercellular adhesion molecules (PECAM+ or VE-cadherin+ EDEVs) may reflect increased endothelial permeability and could be valuable diagnostic and prognostic markers for sepsis.
Study context
- Population
- Patients with sepsis or septic shock and nonsepsis patients; Human umbilical vein endothelial cells (HUVECs) stimulated with TNF-α
- Participants
- 30 / 30 / 42
- Exposure
- Septic shock vs sepsis: circulating platelet endothelial cell adhesion molecule (PECAM)+ and vascular endothelial (VE)-cadherin+ endothelium-derived extracellular vesicles (EDEVs)
- Nonsepsisn = 30
- Sepsisn = 30reference
- Septic shockn = 42
- Outcome
- PECAM+ and VE-cadherin+ EDEVs on admission
- Follow-up
- On admission
Methodological detailsin vitro HUVEC experiments
- In vitro HUVEC experiments
Main finding
As reported in the abstract
Average treatment effect (PECAM+ EDEVs)
98 /μL
95% CI 2–270P = 0.043
Septic shock
Average treatment effect (ATE) (VE-cadherin+ EDEVs)
79 /μL
95% CI 19–171P = 0.004
Septic shock
Observational data: associations, not effects.
Further findings
In vitro HUVEC endothelial permeability and EDEV release
- TNF-α significantly increased EDEV release and endothelial permeability
- Angiopoietin-1 or prostacyclin pretreatment suppressed TNF-α-induced permeability and PECAM+ and VE-cadherin+ EDEV release
- Vascular endothelial growth factor (VEGF) pretreatment increased TNF-α-induced permeability and PECAM+ and VE-cadherin+ EDEV release
- Permeability-modifying reagents did not affect release of E-selectin, ICAM-1 or VCAM-1 EDEVs
Original publication
Endothelium-Derived Extracellular Vesicles Expressing Intercellular Adhesion Molecules Reflect Endothelial Permeability and Sepsis Severity. Anesth Analg. Published 15 July 2024. doi:10.
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