In Vitro Analysis of Platelet Adhesion, Aggregation, and Surface GP1bα Expression in Stored Refrigerated Whole Blood: A Pilot Study
Authors' conclusion
Our study demonstrated significant decreases in measurable platelet count, platelet adhesion, and aggregation under high shear, platelet activation, and surface GP1bα expression between cold-storage days 5 and 19.
Study context
- Population
- Cold-stored whole blood (WB) samples
- Comparison
- Cold-stored WB on storage day 5 vs 12 vs 19
- Outcome
- In vitro platelet adhesion and aggregation over time
Methodological detailsin vitro, pilot, exploratory, serial measurements
- In vitro
- Pilot
- Exploratory
- Serial measurements
Main finding
As reported in the abstract
Platelet adhesion and aggregation under high shear (PFA-100 adenosine diphosphate (ADP)/collagen closure time)
P = 0.04
Further findings
| Outcome | Timepoint 1 | Timepoint 3 | P value |
|---|---|---|---|
| Measurable platelet countTimepoint 3 vs Timepoint 1 · decreased | mean 163 (+ 53) × 109 platelets per liter | mean 107 (+ 32) × 109 | P = 0.02 |
| Peak dense granule release in response to thrombin (platelet activation)Timepoint 3 vs Timepoint 1 · decreased significantly | mean 0.7 (+ 0.3) nmol | mean 0.4 (+ 0.3) | P = 0.05 |
| GP1bα surface expression (flow cytometry)Timepoint 3 vs Timepoint 1 · decreased | mean 232,552.8 (+ 32,887.0) relative fluorescence units | mean 95,133.3 (+ 20,759.2) | P < 0.001 |
Original publication
In Vitro Analysis of Platelet Adhesion, Aggregation, and Surface GP1bα Expression in Stored Refrigerated Whole Blood: A Pilot Study. Anesth Analg. Published 14 April 2023. doi:10.
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