Prognostic implication of downregulated exosomal microRNA (miRNAs) in patients with sepsis: a cross-sectional study with bioinformatics analysis
Authors' conclusion
Among the differentially-expressed miRNAs detected in microarrays, the top four downregulated exosomal miRNAs (hsa-let-7f-5p, miR-331-3p miR-301a-3p, and miR-335-5p) were identified as independent prognostic factors for in-hospital and 90-day mortalities among sepsis patients.
Study context
- Population
- Critically ill adults with sepsis admitted to the ICU, from an ongoing prospective registryvalidation: 35 sepsis, 10 controls
- Participants
- 135 vs 11
- Exposure
- Sepsis patients vs healthy controls
- Sepsisn = 135
- Healthy controlsn = 11reference
- Outcome
- Differential expression of plasma exosomal miRNAs
Methodological detailsbioinformatics analysis, external validation cohort, prospective registry
- Bioinformatics analysis
- External validation cohort
- Prospective registry
Main finding
As reported in the abstract
Differentially expressed exosomal miRNAs
miRNAs significantly changed in sepsis vs healthy controls
25
Upregulated miRNAs
8
Downregulated miRNAs
17
Observational data: associations, not effects.
Further findings
Prediction of in-hospital mortality (AUC)
- hsa-let-7f-5p AUC
- 0.913
- miR-331-3p AUC
- 0.931
- miR-301a-3p AUC
- 0.929
- miR-335-5p AUC
- 0.957
- p < 0.001
- Confirmed in external validation cohort
90-day mortality, high vs low miRNA levels (Kaplan-Meier)
- Significant difference in 90-day mortality between high and low miR-335-5p, miR-301a-3p, hsa-let-7f-5p and miR-331-3p levels
- p < 0.001, log-rank test
KEGG pathway analysis
- Most common pathways for the four miRNAs: PI3K-Akt and mitogen-activated protein kinase (MAPK) signaling
Original publication
Prognostic implication of downregulated exosomal miRNAs in patients with sepsis: a cross-sectional study with bioinformatics analysis. J Intensive Care. Published 3 August 2023. doi:10.
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